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iP & OEiP – a perfect couple to regulate plant development with high spatial resolution. |
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Barbora Pařízková [a, b], Ioanna Antoniadi [c], Michal Karady [b], David J. Poxson [d], Daniel T. Simon [d], Ivan Petřík [a], Miroslav Strnad [a], Ondřej Novák [a], Karel Doležal [a, b], Karin Ljung [c] |
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[a] Laboratory of Growth Regulators, The Czech Academy of Sciences, Institute of Experimental Botany & Palacký University, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic
[b] Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic
[c] Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, 901 83 Umeå, Sweden
[d] Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, 601 74 Norrköping, Sweden
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Cytokinins are plant hormones playing and crucial role in plant development. They are involved in many physiological processes including cell division and differentiation, regulation of activity of apical meristems, senescence delaying or regulating of lateral root development. Since the modulation of plant growth by phytohormones lies on very dynamic interactions of growth regulators, it is of great interest to develop the method with high spatial resolution of hormone delivery within arbitrary developmental frames. Here we present an organic electronic ion pump (OEIP), a device enabling a flow-free and very specific transport of isoprenoid-type cytokinin isopentenyladenin (iP). Using lateral root (LR) architecture as a model system, we estimated the resolution of delivery less than 1 mm, affecting only the OEIP-treated LR with no effect of neighbouring roots. Moreover, while specifically targeting one LR primordia with OEIP, our data demonstrate that the influence of cytokinin in LR development may differ in different stages of LR development. Taken together, OEIP represent a novel, very useful and tunable device of cytokinin treatment which can help to unravel the functions of these hormones in different plant developmental processes. |
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This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic via ERDF-Project “Plants as a tool for sustainable global development” no. CZ.02.1.01/0.0/0.0/16_019/0000827 and by Knut and Alice Wallenberg Foundation ShapeSystems project Grant KAW 2012.0050. Barbora Pařízková was also partially supported by Mobility Support on UP no. CZ.02.2.69/0.0/0.0/16_027/0008482 and by the Internal Grant Agency of Palacký University no. IGA_PrF_2019_018. |
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