Advanced microscopy for plant developmental and cell biology

   

Šamaj J [a], Ovečka M [a], Komis G [a], Šamajová O [a]

   
   

[a] Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University Olomouc, Šlechtitelů 27, 783 71 Olomouc, Czech Republic

   
   

Advanced light-sheet and super-resolution microscopy methods were developed and used in plant science during last decade (Ovečka et al. 2015, 2018; Komis et al. 2015a,b). Living plants are cumbersome samples for advanced imaging especially due to the specific optical properties of cell surfaces and vacuoles as well as specific environmental requirements for undisturbed plant development and growth. Thus, both light-sheet and super-resolution microscopy methods applied to plants have to respect such limiting factors. We developed two reliable and broadly applicable protocols for short-term and long-term bioimaging of living plants using light-sheet microscopy (Ovečka et al. 2015) and structured illumination microscopy (Komis et al. 2015a). We have used these advanced microscopy methods for diverse developmental and cell biological studies showing contribution of secretory vesicles to the oscillatory growth of root hairs, signalling by mitogen-activated protein kinase during cell division, monitoring of microtubule dynamics during plant morphogenesis, dynamic behaviour and interactions of endosomes in root hairs and nuclear changes in diverse root developmental zones and tissues. Most publications were focused on the model plant Arabidopsis but we successfully applied such advanced imaging also on crops such as alfalfa, tobacco and barley.

References:
Komis et al. (2015a) Nat. Protoc. 10, 1248-1263.
Komis et al. (2015b) Trends Plant Sci. 20, 834-843.
Ovečka et al. (2015) Nat. Protoc. 10, 1234-1247.
Ovečka et al. (2018) Nat. Plants 4, 639-650.

   
     This work was supported by ERDF project ‘Plants as a tool for sustainable global development’ (CZ.02.1.01/0.0/0.0/16_019/0000827).

 

 

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9:00 - 17:00

Registration

11:00 - 11:15

Welcome address

 

Danuše Nerudová
Rector, Mendel University in Brno

11:15 - 12:15 Opening Lecture

 

 

 

Dirk Inzé

The pivotal role of Plant Biology in a rapidly changing world

12:15 - 13:15 Keynote Lecture 01

 

 

 

Philip Wigge

Plants in a warming world

13:15 - 14:45  Lunch break & Poster Viewing Sessions
   
14:45 - 15:15 Talk M01
  George Komis
Conditional and developmental rearrangements of the plant cytoskeleton
15:15 - 15:30 Talk M02
  Antonio Pompeiano
Photosynthetic and growth responses of Arundo donax L. plantlets under different oxygen deficiency stresses and reoxygenation
15:30 - 15:45 Talk M03
  Iva Pavlović
Early response of white cabbage (Brassica oleracea var. capitata) to increased salinity: transcriptomic, hormonal and metabolic status

15:45 - 16:00 Talk M04
 

Tereza Dobisová
Non-invasive in planta Monitoring of Chlorophyll Biosynthesis

16:00 - 16:30 Coffee break
   
16:30 - 17:30 Keynote Lecture 02

 

 

David Alabadí

Upstream and downstream events in DELLA-regulated growth and development

17:30 - 17:45 Talk M05
  Helene Robert Boisivon
Thermomorphogenesis during seed development In Arabidopsis and Brassica napus
17:45 - 18:00 Talk M06
  Sahu Pranav Pankaj 
Understanding the response of Arabidopsis under climate change scenarios
18:00 - 18:15 Talk M07
  Jozef Balla 
Auxin flow-mediated competition between axillary buds fine-tuned by other players
18:15 - 20:00   Welcome party, Poster and student talk session
   

 

9:30 - 10:15 Keynote Lecture 03

 

 

Peter Hedden

The role of gibberellin signaling in plant responses to abiotic stress

10:15 - 10:45 Talk T01
  Jan Hejátko

Cytokinins control xylem development via NAC SECONDARY WALL THICKENING PROMOTING FACTORs

10:45 - 11:15 Talk T02
  Marie-Theres Hauser
Metal ion sensing in cell walls
11:15 - 11:30 Talk T03
  Barbora Pařízková
iP & OEiP – a perfect couple to regulate plant development with high spatial resolution
11:30 - 12:00 Coffee break
   
12:00 - 13:00 Keynote Lecture 04

 

 

Miguel A. Blázquez

An ancestral mechanism for the coordination of transcriptional programs

13:00 - 13:30 Talk T04
  Tomáš Werner
Molecular mechanisms controlling cytokinin homeostasis in the endoplasmic reticulum
13:30 - 14:00 Talk T05
  Radomíra Vaňková
The role of cytokinins in optimal defense to abiotic and biotic stresses as well as pathogen attack strategy
14:00 - 15:30   Lunch break & Poster Viewing Sessions
   
15:30 - 16:30 Keynote Lecture 05

 

 

Thomas Schmülling

New chapters from cytokinin action in development and stress defense

16:30 - 17:30 Keynote Lecture 06

 

 

Karin Ljung

Auxin-cytokinin metabolism, transport and signaling during lateral root development

17:30 - 18:00 Coffee break
   
18:00 - 19:00 Keynote Lecture 07

 

 

Aaron Rashotte

Roles of Cytokinin Response Factors in different environmental stress responses

19:00 - 22:00

Conference dinner

9:00 - 10:00 Keynote Lecture 08

 

 

Mark Stitt

Budgeting carbon in a changing environment: Are plants better than bankers and politicians?

 

10:00 - 11:00 Keynote Lecture 09

 

 

Ondřej Novák

Phytohormone profiling at the cellular and subcellular levels

 

11:00 - 11:30

 Coffee break
   
11:30 - 11:45 Talk W01
  Hana Leontovyčová
Can actin depolymerization induce plant resistance?
11:45 - 12:00 Talk W02
  Parisa Rahimzadeh Karvansara

Sulfur metabolism in C4 and C3 plants, Similarities and differences

12:00 - 12:15 Talk W03
  Jan Skalák
Exploring natural variability in multistep phosphorelay for enhanced drought tolerance in plants
12:15 - 12:45 Talk W04
  Timothy O. Jobe

Plant mineral nutrition under changing environment

12:45 - 13:15 Talk W05
  Jozef Šamaj

Advanced microscopy for plant developmental and cell biology

13:15 - 13:45 Talk W06
  Miroslav Ovečka

Mitogen-activated protein kinases as developmental and stress-related modulators in plants

14:00 - 15:00 Lunch
   
15:00 - 16:00 Keynote Lecture 10

 

 

Ashverya Laxmi

Carbon control of shade avoidance responses in Arabidopsis

16:00 - 17:00 Keynote Lecture 11

 

 

Jutta Ludwig-Müller

Plant hormones in biotic interactions

17:00

Closing remarks

  Poster P01
 
Venkata Pardha S. Attuluri
Optimizing clearing methods for deeper imaging of fluorescent protein-expressing plant tissues
  Poster P02
  Kateřina Bělonožníková
The role of organic nitrogen source in plant nutrition
  Poster P03
  Miroslav Berka
Plant-pathogen interaction – proteomics and metabolomics analyses of Phytophthora infection in poplar
  Poster P04
  Hana Habánová
Seed quality under changing environmental conditions
  Poster P05
  Petra Hofmanová
Downregulation of PHOTOTROPIN 1 in Solanum lycopersicum L.
  Poster P06
 

Veronika Hýsková
Could heat shock or oomycete Pythium oligandrum treatment affect the course of viral infection in plants?

  Poster P07
 

Petra Jiroutová
Hormonal analysis of brassinosteroid biosynthetic mutant plants

  Poster P08
  Marek Klemš

Comparison of 14C-activity distribution after 14C-fluoranthene and 14C-glycine uptake by young pea plants

  Poster P09
  Markéta Luklová
A role of plant circadian rhythms as a regulator of plant hormone signaling and ROS signaling
  Poster P10
  Veronika Malých
Cytokinin modulates biotic interaction between Acremonium alternatum and Arabidopsis thaliana
  Poster P11
  Jana Oklešťková
Interactions between steroids in regulation of plant development
  Poster P12
  Helena  Ryšlavá 
What is the effect of seed treatment with Pythium oligandrum on the metabolism of rape plants?
  Poster P13
  Iñigo Saiz-Fernández 
Metabolomic response of Quercus variabilis during Phytophthora cinnamomi infection
  Poster P14
  Eva Nevrtalová
Whole transcriptome profiling of Silene vulgaris: molecular background of the copper tolerance in the non-model plant
  Poster P15
  Tereza Dobisová 
Seeding, the bottleneck of plant science
  Poster P16
  Archna Tiwari 
Carbon control of shade avoidance responses in Arabidopsis
  Poster P17
  Naghani Shekoufeh Ebrahimi 
Study of the involvement of light components pathway (PIF4 and PhyB) in high temperature response in inflorescence of Arabidopsis thaliana

 

  • Plant abiotic stress
  • Biotic interactions
  • Plant signalling
  • Phytohormones
  • Plant growth and development
  • Global change and sustainable agriculture


  

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