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[a] Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University Olomouc, Šlechtitelů 27, 783 71 Olomouc, Czech Republic
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Advanced light-sheet and super-resolution microscopy methods were developed and used in plant science during last decade (Ovečka et al. 2015, 2018; Komis et al. 2015a,b). Living plants are cumbersome samples for advanced imaging especially due to the specific optical properties of cell surfaces and vacuoles as well as specific environmental requirements for undisturbed plant development and growth. Thus, both light-sheet and super-resolution microscopy methods applied to plants have to respect such limiting factors. We developed two reliable and broadly applicable protocols for short-term and long-term bioimaging of living plants using light-sheet microscopy (Ovečka et al. 2015) and structured illumination microscopy (Komis et al. 2015a). We have used these advanced microscopy methods for diverse developmental and cell biological studies showing contribution of secretory vesicles to the oscillatory growth of root hairs, signalling by mitogen-activated protein kinase during cell division, monitoring of microtubule dynamics during plant morphogenesis, dynamic behaviour and interactions of endosomes in root hairs and nuclear changes in diverse root developmental zones and tissues. Most publications were focused on the model plant Arabidopsis but we successfully applied such advanced imaging also on crops such as alfalfa, tobacco and barley.
References:
Komis et al. (2015a) Nat. Protoc. 10, 1248-1263.
Komis et al. (2015b) Trends Plant Sci. 20, 834-843.
Ovečka et al. (2015) Nat. Protoc. 10, 1234-1247.
Ovečka et al. (2018) Nat. Plants 4, 639-650.
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