Can actin depolymerization induce plant resistance?
   

Leontovyčová H [a, b, c], Trdá L [b], Kalachova T [a, b], Nečasová L [b], Dobrev P I [b], Pospíchalová R [b], Malínská K [a], Burketová L [a, b], Valentová O [], Janda M []

   
   

[a] University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague 6, Czech Republic
[b] Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojova 263, 165 02, Prague 6, Czech Republic
[c] Faculty of Science, Charles University in Prague, Faculty of Science, 128 44 Hlavova 2030/8, Prague 2, Czech Republic

   
   

Actin cytoskeleton integrity is necessary for immune response upon pathogen attack, both by providing physical barrier and by its involvement in transport of callose, antimicrobial compounds and cell wall components to the infection site. Regarding these facts it has been generally assumed that desintegration of actin cytoskeleton results in enhanced plant susceptibility to pathogens.
However, we have shown that pharmacological actin depolymerization induces the salicylic acid (SA) signalling pathway which contributes to increased plant resistance. We attempt to investigate this apparent inconsistency and show that the relationship between actin depolymerization and plant resistance is more complex than previously thought. After actin depolymerization we observed high increase in the concentration of SA which in this case is biosynthesized via the isochorismate synthase (ICS) pathway. These effects were observed in two different pathosystems: i) Arabidopsis thaliana x Pseudomonas syringae and ii) Brassica napus x Leptosphaeria maculans. In both pathosystems, actin depolymerization activated the SA pathway, leading to increased plant resistance. Timing of the SA pathway activation is crucial for induction of the resistance. To our best knowledge, we herein provide the first direct evidence that disruption of the actin cytoskeleton can lead to increased plant resistance to pathogens, and that SA is vital to this process.

   
    The work was supported from European Regional Development Fund-Project "Centre for Experimental Plant Biology" (No. CZ.02.1.01/0.0/0.0/16_019/0000738), Czech Science Foundation grant no. 17-05151S and GAUK no. 992416.

 

 

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9:00 - 17:00

Registration

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Welcome address

 

Danuše Nerudová
Rector, Mendel University in Brno

11:15 - 12:15 Opening Lecture

 

 

 

Dirk Inzé

The pivotal role of Plant Biology in a rapidly changing world

12:15 - 13:15 Keynote Lecture 01

 

 

 

Philip Wigge

Plants in a warming world

13:15 - 14:45  Lunch break & Poster Viewing Sessions
   
14:45 - 15:15 Talk M01
  George Komis
Conditional and developmental rearrangements of the plant cytoskeleton
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  Antonio Pompeiano
Photosynthetic and growth responses of Arundo donax L. plantlets under different oxygen deficiency stresses and reoxygenation
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Early response of white cabbage (Brassica oleracea var. capitata) to increased salinity: transcriptomic, hormonal and metabolic status

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Tereza Dobisová
Non-invasive in planta Monitoring of Chlorophyll Biosynthesis

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David Alabadí

Upstream and downstream events in DELLA-regulated growth and development

17:30 - 17:45 Talk M05
  Helene Robert Boisivon
Thermomorphogenesis during seed development In Arabidopsis and Brassica napus
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Auxin flow-mediated competition between axillary buds fine-tuned by other players
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9:30 - 10:15 Keynote Lecture 03

 

 

Peter Hedden

The role of gibberellin signaling in plant responses to abiotic stress

10:15 - 10:45 Talk T01
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Cytokinins control xylem development via NAC SECONDARY WALL THICKENING PROMOTING FACTORs

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Metal ion sensing in cell walls
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iP & OEiP – a perfect couple to regulate plant development with high spatial resolution
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Miguel A. Blázquez

An ancestral mechanism for the coordination of transcriptional programs

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Molecular mechanisms controlling cytokinin homeostasis in the endoplasmic reticulum
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Thomas Schmülling

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Auxin-cytokinin metabolism, transport and signaling during lateral root development

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Budgeting carbon in a changing environment: Are plants better than bankers and politicians?

 

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Ondřej Novák

Phytohormone profiling at the cellular and subcellular levels

 

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  Hana Leontovyčová
Can actin depolymerization induce plant resistance?
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  Parisa Rahimzadeh Karvansara

Sulfur metabolism in C4 and C3 plants, Similarities and differences

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Plant mineral nutrition under changing environment

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Advanced microscopy for plant developmental and cell biology

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Mitogen-activated protein kinases as developmental and stress-related modulators in plants

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Carbon control of shade avoidance responses in Arabidopsis

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Jutta Ludwig-Müller

Plant hormones in biotic interactions

17:00

Closing remarks

  Poster P01
 
Venkata Pardha S. Attuluri
Optimizing clearing methods for deeper imaging of fluorescent protein-expressing plant tissues
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  Kateřina Bělonožníková
The role of organic nitrogen source in plant nutrition
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Plant-pathogen interaction – proteomics and metabolomics analyses of Phytophthora infection in poplar
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  Hana Habánová
Seed quality under changing environmental conditions
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  Petra Hofmanová
Downregulation of PHOTOTROPIN 1 in Solanum lycopersicum L.
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Veronika Hýsková
Could heat shock or oomycete Pythium oligandrum treatment affect the course of viral infection in plants?

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Petra Jiroutová
Hormonal analysis of brassinosteroid biosynthetic mutant plants

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  Marek Klemš

Comparison of 14C-activity distribution after 14C-fluoranthene and 14C-glycine uptake by young pea plants

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  Markéta Luklová
A role of plant circadian rhythms as a regulator of plant hormone signaling and ROS signaling
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  Veronika Malých
Cytokinin modulates biotic interaction between Acremonium alternatum and Arabidopsis thaliana
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Interactions between steroids in regulation of plant development
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  Eva Nevrtalová
Whole transcriptome profiling of Silene vulgaris: molecular background of the copper tolerance in the non-model plant
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Seeding, the bottleneck of plant science
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  Archna Tiwari 
Carbon control of shade avoidance responses in Arabidopsis
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  Naghani Shekoufeh Ebrahimi 
Study of the involvement of light components pathway (PIF4 and PhyB) in high temperature response in inflorescence of Arabidopsis thaliana

 

  • Plant abiotic stress
  • Biotic interactions
  • Plant signalling
  • Phytohormones
  • Plant growth and development
  • Global change and sustainable agriculture


  

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